Si−Si−O 

O−Si−O 

Si-H₂O

Si−O−Si

PBS

Oxygen

None

Nitrogen

Using an incubator for cells

Using a plasma cleaner

It is not necessary to maintain the chip at 37°C

Using your own hands (body temperature)

Because the human brain is enriched in this protein

b and c are both correct

Because endothelial cells synthesize fibronectin

Because fibronectin is very abundant in the extracellular matrix

Because we are using plasma

Because solutions are toxic and we must sterilize them

Because we are handling a microfabricated chip

Because we are going to culture cells in the chip

1. Master mold

2. PDMS pouring and reticulation

3. Release PDMS replica

4. Put in contact for bonding

5. Plasma treatment

6. Inlet drilling

1. Master mold

2. PDMS pouring and reticulation

3. Inlet drilling

4. Release PDMS replica

5. Plasma treatment

6. Put in contact for bonding

1. Master mold

2. PDMS pouring and reticulation

3. Release PDMS replica

4. Inlet drilling

5. Plasma treatment

6. Put in contact for bonding

1. Master mold

2. PDMS pouring and reticulation

3. Release PDMS replica

4. Inlet drilling

5. Put in contact for bonding

6. Plasma treatment