Bridge amplification generates clusters of DNA fragments on the flow cell, enabling the amplification of sequencing signals

The process creates a dense array of identical DNA fragments from each starting template, essential for high-throughput sequencing

Bridge amplification is not used in RNA sequencing, as RNA templates cannot be processed on an Illumina flow cell.

The process creates dense clusters of identical DNA molecules necessary for high signal intensity during sequencing.

During bridge amplification, DNA bends to hybridize with nearby primers, forming a "bridge" structure

Bridge amplification occurs after DNA fragments are attached to the flow cell via complementary adapter sequences.

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DNA fragments are amplified in solution before being deposited onto the flow cell

Each cluster on the flow cell originates from multiple DNA fragments to improve accuracy

Post-translational modifications occur on proteins after transcription, and RNA-Seq does provide accurate information  PTMs

RNA-Seq could only infer PTMs indirectly by identifying transcripts encoding enzymes responsible for such modifications

RNA-Seq detects modifications to RNA molecules   rather than modifications on proteins

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RNA-Seq captures transcriptomic data, focusing on RNA molecules rather than proteins where PTMs occur

The sequence is determined by measuring the length of DNA fragments as they pass through a detector

Each nucleotide has a unique fluorescent signal, allowing the sequence to be read in real time

Fluorescently labeled nucleotides are incorporated into the DNA strand, and their emission signals are captured by a camera

The flow cell generates raw electrical signals, which are converted into sequences without fluorescence

DNA sequencing occurs through a process called "sequencing by synthesis," where nucleotides are added one at a time

The adapter sequences depend on the gene to detect

The genes to detect are synthesized  on the surface of the flow cells by the manufacturer

The sequence is defined by the color fluorescence code as we move across the flow cell line, against the flow. 

The sequence is defined by the color fluorescence code as we move across the flow cell line, towards the flow. 

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The genes to detect are synthesized  on the surface of the flow cells by the client