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202520 - BIO472 - 20812

202520 - BIO472 - 20812

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If the gel is poured too hot, it may damage the casting tray

True

False

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Electrophoresis should be performed at 300V for faster results.

True

False

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What happens if the electrophoresis is run for too long?

The gel will become more solid

The DNA will become undetectable

DNA fragments may run off the gel

100%

The DNA will become more concentrated

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Why is the agarose gel heated in the microwave?

To activate the DNA stain

To denature DNA

To dissolve the agarose powder completely

100%

To sterilize the solution

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Matching Question: Gel Electrophoresis

DNA ladder/standard

Homozygous (+/+) control

Homozygous (-/-) control

UV light or gel imager

TBE/TAE buffer

Voltage supply

Agarose gel

Loading dye

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What is the role of the Alu sequence in this experiment?

It codes for a specific protein

❌

It acts as a restriction enzyme

❌

It is a coding region of the genome

❌

It is a repetitive element used to study polymorphism

✅

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Which method is used to extract DNA from hair follicles in this experiment?

InstaGene matrix with protease digestion

✅

Centrifugation with ethanol precipitation

❌

Chemical lysis with phenol-chloroform

❌

Mechanical grinding

❌

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Matching Exercise: Controls and Experimental Validity

Why is a positive control used in PCR?

Why are multiple samples used in an experiment?

What is the purpose of a negative control in PCR?

What would it indicate if the negative control shows a DNA band after PCR?

Why is it important to include homozygous (+/+), homozygous (-/-), and heterozygous (+/-) controls?

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Match each PCR-related concept with its correct description

Taq polymerase

Primers

PV92 locus

PCR

DNA amplification

Annealing (50-65°C)

Denaturation (94°C)

Thermal cycling

Extension (72°C)

Genomic DNA

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What does "dimorphic" mean in the context of the PV92 Alu insert

The insert is present in all individuals

❌

The insert is either present or absent in individuals

✅

The insert has two different restriction sites

❌

The insert is only found in males

❌

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