B.

They encapsulate individual cells and reagents into droplets

C.

They replace the sequencing step

A.

They increase cell size

D.

They analyze only tumor cells

E. They remove ribosomal RNA

A.

They do not contain messenger RNA

E. They do not contain a genome

D.

They are removed during read alignment

B.

They cannot be sequenced

C.

Their signal is diluted within the average expression of all cells

B. The

abundance of different cell populations in a tissue

E. The

tumor proliferation rate

D. 

10

immune cells types

C. The

three-dimensional structure of proteins

A. The

total number of somatic mutations

B.

Single-cell RNA-seq measures the average gene expression of all cells in a

tissue

C.

Single-cell RNA-seq measures gene expression at the level of individual cells

E.

Both methods analyze only rare cells

A.

Bulk RNA-seq measures only non-coding RNA

D.

Bulk RNA-seq sequences only DNA

D.

Functional annotation

B.

Clustering

C.

Alignment to the reference genome

E.

Differential expression analysis

A. Normalization

E. An

immune reaction

D. A

metabolic pathway

A. An

enzymatic reaction

B. A

cooking recipe mixing several ingredients

C.

Cell division

D. A

matrix describing chromosomal positions

B. A

matrix containing the number of reads assigned to each gene in each sample

E. A

matrix containing expressed proteins

A. A

matrix containing mutations per gene

C. A

matrix describing protein interactions

D.

Erythrocytes

E.

Adipocytes

C.

Epithelial cells

A.

Dendritic cells

B.

Fibroblasts

C.

Determining the full genome sequence of an individual

A.

Determining the three-dimensional structure of proteins

E.

Quantifying only ribosomal RNA

B.

Identifying rare cell populations in a tissue

D.

Measuring the cell division rate

C. A

specific type of metastatic tumor

E. All

circulating blood cells

B. The

collection of cells and structures surrounding the tumor

D. The

genome of cancer cells

A. All

mutations present in a tumor