Haemopoietic stem cells are manipulated and returned to the patient 

Single pluripotential stem cells are isolated from a multipotential population

Mature somatic cells are treated with retroviruses to produce undifferentiated stem cells

Patient specific pluripotent stem cell lines could be generated

Inner cell mass

Epidermal stem cell

Endoderm

Zygote

Producing the same tissues at all axial levels of the neural tube

Multipotent following differentiation

Multipotent during early migration

Restricted in cell fate prior to migration

Make and secrete uncalcified extracellular matrix

Maintain the calcification of bone matrix over a period of time

Breakdown and resorb calcified extracellular matrix

Smooth, cardiac, skeletal

Smooth, skeletal, cardiac

Cardiac, skeletal, smooth

Skeletal, smooth, cardiac

Blood vessels of the abdomen

Long bones of the limbs

Melanocytes of the skin

Epithelium of the bowel

At puberty

During late gestation

Immediately after birth

At menopause

Removal of the region of ectoderm to see if there is decreased outgrowth in the nasal process

RNA sequencing of the ectoderm to identify the genes expressed in the ectodermal region of interest 

Tissue culture of the region of ectoderm with nasal process mesenchyme in vitro

Immunohistochemistry to look at protein expression in the nasal process tissue

The motor neurons will differentiate into other neuronal types such as sensory neurons

The axons of the motor neurons will continue to extend until they find some muscle

The motor neurons in the spinal cord will find other targets to synapse on

More motor neurons will undergo apoptosis than normal

Vertebrae and pelvis

Ribs

Sternum

Femur