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BIO1011 - Blueprints for Life - Clayton S1 2025
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Write a brief reflection on the technical or academic skills that you have developed during this lab session.Things to consider:
- Briefly describe the activities in the lab session. What was the outcome?
- How did you feel undertaking the lab activities? Why do you think you felt that way?
- Name some things that you believe you've done well during this lab session. What do you think you did poorly on and how can you improve?
- What did you learn from today's lab? How was this lab linked to the theory?
From the data collated in the gene expression component of this lab:a) What features do you want the AraPβ regulatory region to have from a commercial perspective? Justify your answer.b) If you were working as a biomedical engineer for this company, which mutant would you recommend has the most commercial value? Justify.c) Using the genetic information provided for the AraPβ1 and AraPβ4 reporter constructs (nucleotide sequences), describe how their observed phenotype can be attributed to a mutation in the respective genotype of these two reporter constructs. To determine this, you need to:
- compare the phenotype in the mutants with the phenotype of the wildtype, for each reporter construct, to determine how the mutation presents, phenotypically.
- check for mutations in the regulatory regions of the gene - this would include the AraC binding site and the promoter region. This is so that you can locate a genetic mutation that is responsible for a difference in the phenotype.
- predict the impact of these genetic mutations on the transcription of the reporter gene and relate this to the phenotype observed in the lab.
From the data collated from your DNA profiling of the two mothers and two children:a) Which baby belongs to which mother? Explain how you determined this.b) Given the genotypes you recorded for each of the four individuals under investigation in the DNA profiling, can you determine the genotype of the father? Explain.
Prelab Activity
Upload your prelab activity here.Lab 4 Results
Save your lab results! Upload your Lab 4 results here for future reference.Choose one of the following:- Best way: Transcribe the whiteboard data into the tables in your results sheet Word document. This makes it easier to use for your subsequent assignments.
- Quick way: Take a photo of the whiteboard containing all results.
Which of the following statements is correct regarding agarose gel electrophoresis?