Shown below is a Lineweaver-Burk (double-reciprocal) plot for an enzyme-catalysed reaction.

What is the Km value for this reaction? 

(Type your answer in units of mM but do not type the units or any other characters except the number itself.)

Shown below is a binding curve for a drug binding to a single binding site on a target protein.

Using information from this curve, estimate the concentration of the drug required such that 90% of the protein molecules will be bound to the drug.

Your answer should be given in units of nanomolar (nM).

Type only numbers (do not type the units or any other additional characters)

Hint: if you're struggling here because the graph doesn't go high enough, think about what information you can glean from the graph and take it from there.

Shown below (left) is a hydropathy plot for a polytopic integral membrane protein.

Also shown (right) are four topology diagrams (A-D) for integral membrane proteins.

Red arrows indicate the positions at which lysine residues are readily labelled with a chemical reagent added to the extracellular medium.

Which topology diagram is most consistent with the hydropathy plot and lysine labelling pattern shown?

Shown below are the binding curves for wild type and mutant forms of myoglobin.

Which of the following can best explain the effect of the mutation on oxygen binding by myoglobin?

The graph below shows data for the fractional saturation of a protein with varying concentrations of ligand.

Using the graph and your knowledge of Kd, determine the concentration of ligand (in nM; nanomolar) required to achieve 1% of protein bound.

The image below shows part of a structure of a protein, with several amino acids highlighted in ball and stick representation.

Which one statement concerning this region of the structure is true?

Shown below is a binding curve for a drug binding to a single binding site on a target protein.

Using information from this curve, estimate the concentration of the drug required such that 90% of the protein molecules will be bound to the drug.

Your answer should be given in units of nanomolar (nM).

Type only numbers (do not type the units or any other additional characters)

Hint: if you're struggling here because the graph doesn't go high enough, think about what information you can glean from the graph and take it from there.

Shown below are the binding curves for wild type and mutant forms of myoglobin.

Which of the following can best explain the effect of the mutation on oxygen binding by myoglobin?

Shown below (left) is a hydropathy plot for a polytopic integral membrane protein.

Also shown (right) are four topology diagrams (A-D) for integral membrane proteins.

Red arrows indicate the positions at which lysine residues are readily labelled with a chemical reagent added to the extracellular medium.

Which topology diagram is most consistent with the hydropathy plot and lysine labelling pattern shown?

An enzyme, E, displays negative cooperativity (allosteric behaviour) for its substrate, S, in the presence of a modulator, M.

Which curve below (A, or B) represents the dependence of the initial velocity of E on the concentration of S in the presence and absence of M.