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BCH2011 - Structure and function of cellular biomolecules - S1 2025

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One strand of a DNA duplex has the sequence:

5′-CCATTTCGTC-3′

Type the sequence of the complementary strand in the 5′ to 3′ direction.

Be careful not to type any spaces or additional characters. For example, if you think the answer is 5'-AGCC-3', simply type AGCC.

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Compare the two DNA duplexes shown below.

Duplex 1                  Duplex 2

5′-CTAGCTCAAG-3′          5′-GATCGCATGTTC-3′

3′-GATCGAGTTC-5′          3′-CTAGCGTACAAG-5′

Which of the following statement(s) is/are correct?

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You are preparing a tube containing 0.025 μmol of phosphate in a total volume of 5.0 mL.

What volume (μL) of 5 mM stock phosphate solution do you need to add into the tube?
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5 mL of a stock solution of 250 mM Tris buffer is diluted to a final volume of 25 mL.

What is the final concentration of the diluted Tris buffer (in units of mM)?

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You took 3 mL of a stock solution of sodium phosphate (25 mM) and mixed it with 12 mL of water to make solution A.

You then took 1 mL of a solution A and mixed it with 1 mL of water to make solution B. 

You then took 1 mL of a solution B and mixed it with 4 mL of water to make solution C.

Calculate the concentration (in mM) of sodium phosphate in each of these solutions.

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How many micromoles (μmol) of ATP are present in 6 mL of 5 mM ATP solution?

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An experiment was performed in which 250 mL of 0.7 M urea was used.

Calculate the number of millimoles (mmol) of urea contained in this sample.

Note: Be careful of your units!

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An experiment requires you to create a stock buffer containing 0.2 M of NaCl in a total volume of 550 mL.

How many grams (g) of solid NaCl are required to prepare this stock solution?

Note: Your answer should be to two decimal places, e.g. 4.31

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A patient has been admitted to hospital and a blood test has shown his glucose level is 45 mg per 55 mL.

Calculate the molarity (in mM, to two decimal places) of the glucose in the patient's blood.

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A sample contains 700 μg of NaOH.

How many milligrams (mg) is this?

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