Uncouplers

Uncouplers increase the permeability of the inner mitochondrial membrane to protons which allows protons to diffuse back across the membrane and so disrupt the proton gradient. 

Based on your experimental observations, the effect of this will be:

Energy Transformation

The major purpose of the electron transport chain is to provide the energy for the synthesis of ATP from ADP and inorganic phosphate (Pi).

The energy is provided by:

Rotenone inhibits the flow of electrons from FMN to coenzyme Q. Discuss how this affects oxidative phosphorylation.

Why is it important that the inner mitochondrial membrane is impermeable to protons?

A negative change in free energy means there is energy available for use.

Oxidative phosphorylation is the coupling of two distinct processes.

What is the final electron acceptor of the electron transport chain?

Oxidative phosphorylation is dependent on an intact inner mitochondrial membrane.