Uncouplers

Uncouplers increase the permeability of the inner mitochondrial membrane to protons which allows protons to diffuse back across the membrane and so disrupt the proton gradient. 

Based on your experimental observations, the effect of this will be:

Energy Transformation

The major purpose of the electron transport chain is to provide the energy for the synthesis of ATP from ADP and inorganic phosphate (Pi).

The energy is provided by:

To make 1 ATP molecule, 10 protons must be imported through the F₀ component of the ATP synthase.

Total yield of ATP from Glucose via aerobic glycolysis and TCA cycle is always 32.

F₀ is located in the: 

ATP synthase consists of F₁ and F₀ components.

F₁ is located in the:

Protons flow back into the matrix through: 

What is the final electron acceptor of the electron transport chain?