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BIOL 1110 - Principles of Biology LAB (Winter 2025, Sonnenfeld)
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In this lab, you conducted experiments using fluorescence to determine the melting temperature of double-stranded DNA containing different proportions of Watson-Crick base pairs. Based on your prediction, would the following statement True or False?
The
AT-rich DNA lost fluorescence at the lowest temperature suggesting that it had
the
highest melting temperature.Between the two strands of a DNA segment the nitrogen bases are held together by _____.
In this lab, you conducted experiments using fluorescence to determine the melting temperature of double-stranded DNA containing different proportions of Watson-Crick base pairs. Based on your prediction, would the following statement be True or False?
The GC-rich DNA lost fluorescence at the lowest temperature suggesting that it was the most stable at high temperature.