Once you finish purifying your PCR product (Part B), the Agarose gel should be finished running , meaning you can analyse your PCR reactions.
Under which of the following conditions would you need to stop using your own sample for part C and D, and collect a backup sample from your TA instead? (Select all that apply)
Assume that if not mentioned, the -DNA PCR sample does not show any bands of the expected size.

Question

Once you finish purifying your PCR product (Part B), the Agarose gel should be finished running , meaning you can analyse your PCR reactions. 
 Under which of the following conditions would you need to stop using your own sample for part C and D, and collect a backup sample from your TA instead? (Select all that apply) 
 Assume that if not mentioned, the -DNA PCR sample does not show any bands of the expected size.

Accepted Answer

Only DNA fragment(s) of the incorrect size are observed in your +DNA reaction.

Accepted Answer

No DNA fragments are observed in your +DNA reaction.

Accepted Answer

A band of the expected size is seen in your +DNA sample, but so are other sized fragments. The band of the expected size is approximately as bright or is duller than other bands.

Answer

A band of the expected size is seen in your +DNA sample, but so are other sized fragments. The band of the expected size is significantly brighter than all other bands.

Answer

Regardless of your +DNA result, you see a band of the expected size in your -DNA reaction.

Answer

You see a single band in your +DNA lane of the expected size.

Crowdly

Once you finish purifying your PCR product (Part B), the Agarose gel should be f...

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