Following surgical resection, samples of the tumour tissue and adjacent normal colon tissue were fixed in formalin and sectioned into thin slices for histological analysis. The tissue sections were stained with haematoxylin and eosin (H&E) and examined by a pathologist using light microscopy to assess tissue architecture and cellular morphology. Representative histological images from the patient’s normal colon tissue and tumour tissue are shown in Figure 1.

Figure 1. Histological analysis of patient-derived normal colon tissue (A) and colorectal tumour tissue (B) stained with haematoxylin and eosin (H&E).