Refer to Figure 1 , which shows an amplification plot from a real-time PCR (qPCR) assay. In clinical diagnostic microbiology, how does this plot assist in determining the initial quantity of the target pathogen's DNA in a patient sample?

Figure 1: An example of an amplification plot

Question

Refer to  Figure 1 , which shows an amplification plot from a real-time PCR (qPCR) assay.  In clinical diagnostic microbiology, how does this plot assist in determining the initial quantity of the target pathogen's DNA in a patient sample? 
  
 Figure 1: An example of an amplification plot

Answer

By assessing the number of primers used in the qPCR reaction

Answer

By counting the total number of thermal cycles completed during the reaction

Answer

By assessing the cycle threshold (Ct) value

Answer

By interpreting the shape of the amplification curve to estimate the starting DNA concentration

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Refer to Figure 1 , which shows an amplification plot from a real-time PCR (qPC...

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