Shown below is a DNA duplex sequence, including both the coding (top) and non-coding (bottom) strands.
Which one of the following pairs of oligonucleotide primers would be most suitable for amplifying (by PCR) the region of this duplex shaded in yellow?
[Note: You are not expected to estimate Tm values for these primers. If they have the correct sequence to anneal in the correct position, you should assume that they will.]

Question

Shown below is a DNA duplex sequence, including both the coding (top) and non-coding (bottom) strands.  
 Which  one  of the following pairs of oligonucleotide primers would be most suitable for amplifying (by PCR) the region of this duplex shaded in yellow? 
 [Note: You are not expected to estimate Tm values for these primers. If they have the correct sequence to anneal in the correct position, you should assume that they will.]

Answer

Forward primer: 5'-GAGTAATCCCATGGGAAGCG-3' 
 Reverse primer: 5'-TAGTACACGGGCCGAGGCAG-3'

Answer

Forward primer: 5'-CTCATTAGGGTACCCTTCGC-3' 
 Reverse primer: 5'-GACGGAGCCGGGCACATGAT-3'

Answer

Forward primer: 5'-GAGTAATCCCATGGGAAGCG-3' 
 Reverse primer: 5'-ATCATGTGCCCGGCTCCGTC-3'

Answer

Forward primer: 5'-CTCATTAGGGTACCCTTCGC-3' 
 Reverse primer: 5'-ATCATGTGCCCGGCTCCGTC-3'

Answer

Forward primer: 5'-CTCATTAGGGTACCCTTCGC-3' 
 Reverse primer: 5'-TAGTACACGGGCCGAGGCAG-3'

Answer

Forward primer: 5'-GAGTAATCCCATGGGAAGCG-3' 
 Reverse primer: 5'-GACGGAGCCGGGCACATGAT-3'

Crowdly

Shown below is a DNA duplex sequence, including both the coding (top) and non-co...

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