In attempting to define the subunit composition of an unknown protein, you decide to subject the protein to SDS-PAGE in the presence or absence of the reducing agent DTT. The results of this analysis are shown below.
Lane 1: molecular weight marker ladder.
Lane 2: protein sample without DTT
Lane 3: protein sample with DTT.

From this analysis, what is the subunit composition of the original native protein ?

Question

In attempting to define the  subunit composition  of an unknown protein, you decide to subject the protein to  SDS-PAGE  in the presence or absence of the reducing agent DTT. The results of this analysis are shown below. 
 Lane 1: molecular weight marker ladder. 
 Lane 2: protein sample  without  DTT 
 Lane 3: protein sample  with  DTT. 
  
 From this analysis, what is the  subunit composition of the original native protein ?

Answer

Two subunits. One ~150 kDa and one ~55 kDa, joined by disulphide bonds.

Answer

Four subunits. Two (joined by disulphide bonds) ~55 kDa each and two (joined by disulphide bonds) ~75 kDa each.

Answer

Three subunits. One ~55 kDa and two (joined by disulphide bonds) ~75 kDa each.

Answer

Two subunits. One ~55 kDa and one ~75 kDa, joined by disulphide bonds.

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In attempting to define the subunit composition of an unknown protein, you dec...

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