To minimise unintended genome damage, a gene-editing
strategy must ensure that nuclease activity is restricted to a single,
predefined genomic site. In zinc finger nuclease–based approaches, this
specificity is achieved through a modular DNA-binding architecture.

Which component of a ZFN construct is primarily responsible
for recognising and binding the target DNA sequence?

Question

To minimise unintended genome damage, a gene-editing
strategy must ensure that nuclease activity is restricted to a single,
predefined genomic site. In zinc finger nuclease–based approaches, this
specificity is achieved through a modular DNA-binding architecture.

Which component of a ZFN construct is primarily responsible
for recognising and binding the target DNA sequence?

Accepted Answer

An
array of zinc finger motifs, each engineered to bind a defined three-base-pair
DNA sequence

Answer

A
complementary RNA molecule that guides the nuclease to its genomic target

Answer

The
catalytic nuclease domain, which confers sequence-specific DNA recognition

Answer

A single
three base-pair DNA molecule that guides the nuclease to its genomic target

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