You have a cell suspension for which you need to determine the cell concentratio...
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You have a cell suspension for which you need to determine the cell concentration. You have stained your cells with trypan blue, using 20μl of cell suspension and 40μl of trypan blue. Trypan blue stains dead cells blue, while live cells exclude this dye.
Image failed to load: Example haemocytometer grid.
Your counts for each of the outer squares are:
- Viable: 83; Dead: 36
- Viable: 79; Dead: 50
- Viable: 79; Dead: 48
- Viable: 86; Dead: 47
What is the percentage of viable cells in your sample? Answer to 1 decimal place.
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