The conversion of 2-phosphoglycerate to phosphoenolpyruvate by enolase illustrates metal ion catalysis effectively. Which statement is accurate?

Question

Answer

Glycine is essential for catalysis

Answer

The carboxyl group of 2-phosphoglycerate is coordinated by two magnesium ions within the active site

Answer

Lysine (K345) does not act as a general base

Answer

Magnesium ions are not involved in this catalysis

Answer

During catalysis, an intermediate, so-called Tricarbanion intermediate, is generated

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