A researcher plans to use zinc finger
nucleases (ZFNs) to introduce a precise
double-strand break at a specific site in a gene for genome editing.
They know that ZFNs rely on DNA-binding
domains fused to the FokI nuclease , which only cleaves DNA when
properly dimerized.

Which strategy best explains how ZFNs achieve site-specific DNA cleavage ?

Question

A researcher plans to use  zinc finger
nucleases (ZFNs)  to introduce a  precise
double-strand break  at a specific site in a gene for genome editing.
They know that ZFNs rely on  DNA-binding
domains fused to the FokI nuclease , which only cleaves DNA when
properly dimerized.

Which strategy best explains how ZFNs achieve  site-specific DNA cleavage ?

Accepted Answer

Two
separate ZFN proteins are designed to bind  opposite sides of the target site ,
allowing the FokI nucleases to dimerize and cut the DNA

Answer

A
single ZFN protein with one FokI monomer binds the DNA and cleaves it on its
own

Answer

Two
separate ZFN proteins are designed to bind  directly to same region of the target cleavage
sites , allowing the FokI nucleases to dimerize and cut the DNA

Answer

A
single ZFN protein binds the target site and recruits a pre-formed FokI dimer
to cleave the DNA

Crowdly

A researcher plans to use zinc finger nucleases (ZFNs) to introduce a precis...

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