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When setting up the colorimetric assay, in what order should the sample and reag...

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When setting up the colorimetric assay, in what order should the sample and reagents be added to each well?

1. Sample, 2. Diluted Assay Probe, 3. Assay Enzyme Mix.

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1. Assay Enzyme Mix, 2. Diluted Assay Probe, 3. Sample.

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1. Sample, 2. Assay Enzyme Mix, 3. Diluted Assay Probe.

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1. Assay Enzyme Mix, 2. Sample, 3. Diluted Assay Probe.

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