You find that over-expression of the TOX3 transcription factor gene in the limb bud mesenchyme of mouse produces stunted limbs by E14.5. What method could you use to test if ectopic expression of the gene has the same or similar effect in the chicken embryo limb bud mesenchyme?

Question

You find that over-expression of the  TOX3  transcription factor gene in the limb bud mesenchyme of mouse produces stunted limbs by E14.5. What method could you use to test if ectopic expression of the gene has the same or similar effect in the chicken embryo limb bud mesenchyme?

Answer

In ovo  electroporation of  RCAS virus expressing TOX3  into the chicken limb bud

Answer

In ovo  electroporation of  Tol2  plasmid expressing  TOX3  into the chicken limb bud

Answer

In ovo  electroporation of  TOX3  shRNAs into the chicken limb bud prior to bud outgrowth

Answer

In ovo  electroporation of Cas9 and  TOX3  guide RNAs into the chicken limb bud

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You find that over-expression of the TOX3 transcription factor gene in the lim...

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