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You've performed a haemocytometer count and have determined that there are 1.92 ...

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You've performed a haemocytometer count and have determined that there are 1.92 × 108 cells/ml in your 2 ml of cell suspension.

In the MHC restriction of T cells experiment you will need to make up a 1 ml aliquot of 1 × 106 cells.  You will need to remove μl of cell suspension and add μl of buffer to make up this cell suspension.

(Answer to 2 decimal places.)

Hint: If you are not sure how to complete this calculation check the C1V1 = C2V2 video in the Numbers, graphs and calculations eBook.

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