In the original experiment from Calvin, Benson and Bassham, why were aliquots of the Chlorella culture let out into boiling ethanol?

Question

Answer

To accelerate CO2 assimilation by increasing the temperature of the sample.

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To ensure that all proteins would be denatured, thus stopping any enzymatic reactions from continuing to take place until the samples could be processed and analyzed.

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To ensure that no algae were left alive, which could have caused a biological hazard in the lab.

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To ensure that all photosystems would remain intact.

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In the original experiment from Calvin, Benson and Bassham, why were aliquots of...

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