In the process of creating vaccines, pathogens of infectious diseases are being attenuated. What is the essence of the attenuation process?

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Artificial reduction of virulent properties of pathogens

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Discovering antigenic determinants of the main antigens of the pathogen

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Reduction of immunogenicity of the pathogen

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Inactivation of pathogens while preserving the antigenic structure of cells

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Isolation of protective antigens from microbial cells

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