Researchers are attempting to correct a disease-causing mutation using
CRISPR in mature neurons taken from an adult patient. They plan to use homology-directed repair (HDR) with a
donor DNA template to precisely replace the faulty sequence.

However, the experiment produces very few
successfully edited cells.

What is the most
likely explanation for the poor editing efficiency?

Question

Researchers are attempting to correct a disease-causing mutation using
CRISPR in mature neurons taken from an adult patient. They plan to use  homology-directed repair (HDR)  with a
donor DNA template to precisely replace the faulty sequence.

However, the experiment produces very few
successfully edited cells.

What is the  most
likely explanation  for the poor editing efficiency?

Accepted Answer

HDR
repair mechanisms operate inefficiently in cells that are not actively dividing

Answer

Donor
DNA templates cannot integrate into human cells

Answer

HDR
requires meiotic cell division to function correctly

Answer

The
guide RNA cannot locate a sufficiently unique DNA target sequence in neuronal
DNA

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Researchers are attempting to correct a disease-causing mutation using CRISPR i...

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