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After using dideoxy nucleotides (ddNTPs) in the chain termination (Sanger) metho...
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After using dideoxy nucleotides (ddNTPs) in the chain termination (Sanger) method for sequencing a DNA fragment, you observe the following:
Image failed to load: sequencing gel
Based on the sequencing gel shown above, where the arrow indicates the direction of electrophoresis, which one of the following is the sequence of the original strand of DNA (the strand that was sequenced, that is, was the template for the sequencing reaction)?
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