The Bradford protein assay, which uses Coomassie Brilliant Blue, primarily relies on which principle to estimate protein concentration?

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Answer

The dye binds to amino groups in proteins via covalent bonds, producing a fluorescent signal

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The dye breaks proteins down in their individual amino acids which can be separated via gel electrophoresis

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The dye forms a complex with certain amino acids, leading to a shift in absorbance maximum from 465 nm to 595 nm

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The assay measures protein concentration by monitoring the rate of protein hydrolysis catalysed by the dye

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The dye interacts with certain amino acids, causing a spectral shift that can be measured at 595 nm

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