A molecular biologist performed a polymerase chain

reaction (PCR) experiment and obtained the PCR product shown below in Figure 2.

Enzyme X cleaves this PCR product at the two positions indicated by the red

arrows. The asterisk indicates the location where a DNA probe has been designed

for usage in a Southern blot.

Figure 2: Schematic diagram of a PCR product.

How many bands will appear on a Southern blot after

the PCR product is cleaved?