You have expressed human insulin in Escherichia coli and then lysed the cells....
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You have expressed human insulin in Escherichia coli and then lysed the cells. You are going to purify the insulin from the bacterial proteins by passing the protein lysate over an immunoaffinity column with a Bio-Gel® support (beads). What needs to be adsorbed to the support in order for your purification to be successful?
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