You wish to purify acetylcholinesterase from a sample using immunoaffinity chromatography with a column that contains Reacti-Gel® as a support (beads). You have appropriately set up your column to purify the acetylcholinesterase. You pass your sample over the column and are confident the acetylcholinesterase has been retained on the support.
Which one of the following statements best describes the interaction between acetylcholinesterase and the support at this point in the assay?

Question

You wish to purify acetylcholinesterase from a sample using immunoaffinity chromatography with a column that contains Reacti-Gel® as a support (beads). You have appropriately set up your column to purify the acetylcholinesterase. You pass your sample over the column and are confident the acetylcholinesterase has been retained on the support.  
 Which one of the following statements best describes the interaction between acetylcholinesterase and the support at this point in the assay?

Answer

There is an antigen-antibody reaction between acetylcholinesterase in the sample and anti-acetylcholinesterase antibodies adsorbed to the support.

Answer

There is an electrostatic (charge) interaction between the acetylcholinesterase and the Reacti-Gel® support beads.

Answer

Acetylcholinesterase is a large protein and will be retained by the Reacti-Gel® beads as they create gaps that only small proteins can pass through.

Answer

The Reacti-Gel® support beads are immunogenic and bind to the acetylcholinesterase.

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