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Q9. What would happen if the column was allowed to run dry?

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Q9. What would happen if the column was allowed to run dry?

It would cause the flow to entirely stop, halting the experiment

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It would cause the beads to form covalent bonds with the proteins

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It would lead to the proteins mixing together and reversing their elution order

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It would cause cracks, which would potentially lead to "tunnels" through the column, preventing accurate separation

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