The ANS can be considered as a two neuron chain because

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The CNS is connected to the target tissue through both sympathetic and parasympathetic divisions

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Both the brain and the spinal cord parts of the CNS send efferent input to target tissues

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The CNS is connected to the target tissue through two successive sets of neurons

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The CNS and target tissue are linked through both afferent and efferent neurons

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The CNS is connected to the target tissue through preganglionic or postganglionic nerve fibes

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