Ion exchange chromatography separates proteins according to their net charge. Why is pH an important consideration during this technique?

Question

Ion exchange chromatography  separates proteins according to their net charge. Why is pH an important consideration during this technique?

Answer

Because it will ensure that all proteins carry a net negative charge

Answer

Because it will ensure that all proteins carry a net positive charge

Answer

Because it will ensure the same charge across all proteins being separated

Answer

Because it will affect the net charge of the proteins being separated

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