You have transformed some E. coli with a plasmid vector (pCOOKIE) containing ...
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You have transformed some E. coli with a plasmid vector (pCOOKIE) containing an ampicillin resistance gene.
You perform the transformation as follows:
- 10 µL of a 10 µg/mL stock of plasmid is added to the transformation tube.
- The total volume in this tube is 100 µL.
- All 100 µL from the transformation tube is spread onto the agar plate.
The plates are incubated and the results are shown in the table below:
| Petri dish (plate) | pCOOKIE added | No.colonies |
|---|---|---|
| Nutrient agar | No | > 300 |
| Nutrient agar + ampicillin | No | 0 |
| Nutrient agar + ampicillin | Yes | 150 |
Using the results shown in the table and the transformation efficiency formula below, calculate the transformation efficiency for pCOOKIE in these cells.
Image failed to load: Transformation efficiency equation
Give your answer as the nearest whole number (no decimal points), figures only, no units.
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