Researchers aim to silence a disease-causing allele that
produces a toxic protein by introducing a site-specific double-strand break in
the gene. No donor DNA is supplied, as the goal is to disrupt gene function
rather than repair it.

Which DNA repair mechanism is intentionally relied upon in
this approach, and what makes it suitable for this purpose?

Question

Researchers aim to silence a disease-causing allele that
produces a toxic protein by introducing a site-specific double-strand break in
the gene. No donor DNA is supplied, as the goal is to disrupt gene function
rather than repair it.

Which DNA repair mechanism is intentionally relied upon in
this approach, and what makes it suitable for this purpose?

Accepted Answer

Error-prone
end joining that often generates insertions or deletions leading to gene
knockout

Answer

Homology-dependent
repair that enables accurate correction of the mutation

Answer

High-accuracy
repair that preserves the native coding sequence

Answer

Template-driven
DNA synthesis that accurately restores the original sequence

Crowdly

Researchers aim to silence a disease-causing allele that produces a toxic prote...

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