In diagnostic PCR, samples often contain DNA from both normal microbiota and pathogens. What is the critical component of the PCR reaction that addresses this challenge, and what is its role?

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The denaturation temperature — it ensures uniform melting of DNA strands

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The extension time — it controls DNA synthesis efficiency

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The primers — they provide specificity by binding to the target DNA sequence

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The buffer composition — it maintains optimal pH during the reaction

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In diagnostic PCR, samples often contain DNA from both normal microbiota and pat...

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