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A researcher extracts protein from cultured hepatocytes (i.e. liver cells) and treats the protein sample with sodium dodecyl sulphate (SDS) and β-mercaptoethanol (BME). The protein sample is loaded onto a polyacrylamide gel and a western blot is performed as per the steps described in the relevant online learning module (OLM 6.2). The researcher uses a primary antibody that specifically binds to the N-terminal region of acyl-CoA dehydrogenase.
Examine the western blotting results shown in the image above (A, B, C). Which result would you expect to obtain for this experiment?
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