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Los pasos para la tinción de Ziehl Neelsen son:
Carbol fucsina, calentar 5 min, alcohol acetona, lugol, azul de metileno.
Carbol fucsina, calentar 5 min, alcohol acido, azul de metileno.
Carbol fucsina, sin calentar, acido sulfúrico, azul de metileno.
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