Q1. 

In the laboratory classes in weeks seven and

eight, you used western blotting to detect the MFN2 protein in a patient sample.

The expected western blot result is shown in Figure 1. Complete the steps below

(A-B) to upload and interpret your western blot results.

Figure 1.

Expected western blot result for detection

of MFN2 protein in patient sample. 1 = Precision Plus Kaleidoscope Prestained

Protein Standard, 2 = patient protein lysate.

A) RESULTS UPLOAD:

Upload a photograph of your

western blot result to the file upload box below.

B) RESULTS INTERPRETATION:

Compare your western blot

result to the expected result shown in Figure 1. Did your western blot produce

the expected result? (YES/NO) (4 marks)

If YES, proceed to the next question (Q2).

If NO, explain how your western blot result differs from the

expected result and suggest one or more sources of error or procedural issues

that could explain this difference. (2-3 sentences)