A researcher wants to introduce a targeted
double-strand break in DNA to stimulate genome editing in human cells.
They are considering several programmable gene-editing technologies and need to
choose one capable of generating this type of DNA damage.

Which conclusion best reflects the capabilities
of the available tools?

Question

A researcher wants to introduce a  targeted
double-strand break in DNA  to stimulate genome editing in human cells.
They are considering several programmable gene-editing technologies and need to
choose one capable of generating this type of DNA damage.

Which conclusion best reflects the capabilities
of the available tools?

Accepted Answer

Zinc
Finger Nucleases, TALENs, and CRISPR–Cas9 can all be engineered to create
targeted double-strand breaks in DNA.

Answer

Only
CRISPR–Cas9 can generate targeted double-strand DNA breaks in cells

Answer

Double-strand
DNA breaks cannot be precisely engineered within dividing cells

Answer

Zinc
Finger Nucleases and TALENs can generate double-strand breaks, but CRISPR–Cas9
cannot

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A researcher wants to introduce a targeted double-strand break in DNA to stim...

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