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The sequence below represents the coding DNA strand of the start of a gene, including part of the 5' untranslated region.
(5')...CCGGAAGGAGGTAACCGCAATGACCGGGCCTTTGAACC...(3')
In studying disease variants, a nucleotide mutation of T→A was detected in the second base of the fifth codon. What effect would this mutation have?
(When considering your answer, assume standard initiation of translation is numbered as codon 1.)