Following a mutagenesis screen where male mice were exposed to the chemical mutagen ENU several embryos with different limb defects were identified after 3 generations of mating. How would the genes responsible for the limb phenotypes be identified?

Question

Following a mutagenesis screen where male mice were exposed to the chemical mutagen ENU several embryos with different limb defects were identified after 3 generations of mating.  How would the genes responsible for the limb phenotypes be identified?

Accepted Answer

Sequencing the genome of an embryo with the defect compared to a wild type littermate

Answer

Sequencing of tissue from the male mouse exposed to ENU

Answer

Sequencing of offspring in the first generation following mating

Answer

Backcrossing of the animals to achieve homozygosity

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Following a mutagenesis screen where male mice were exposed to the chemical muta...

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