Question 8: Experimental scenario [5 marks] Clinicians have identified a cohor...
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Question 8:Experimental scenario [5 marks]
Clinicians have identified a cohort of human patients with abnormally small kidneys that have a mutation in a gene named PODRICK. The podrick gene is highly conserved in zebrafish. The clinicians approach you for a collaboration to determine whether mutation of podrick can cause kidney defects.
Firstly, you want to know whether podrick protein is present in the developing kidney.
8a) What method could you use to visualise whether podrick is expressed in the developing kidney in zebrafish? (2 marks)Your results showed that the podrick protein is expressed in the developing kidney in zebrafish. You decide to test whether this gene has a potential role during development by transiently knocking down podrick expression in zebrafish. 8b) Name a method you would use to transiently knock down podrick gene expression. (1 mark)8c) How you would examine whether kidney development had been affected by reducing podrick expression (i.e., the consequences of the knockdown)? (2 marks).Want instant access to all verified answers on learning.monash.edu?
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