A researcher

performed a restriction fragment length polymorphism (RFLP) experiment to

identify the presence of a G or a C in a specific locus of the human genome. To

do this he performed a polymerase chain reaction (PCR) experiment to amplify a

250 bp PCR product. An enzyme called A2B was then used to digest the PCR

product. If a G was present at the specific location of interest the PCR

product would be cleaved into two fragments of 150 bp and 100 bp. If a C was

present, the PCR product would remain intact. The results are shown in Figure 1

below.

Figure 1:

  Agarose gel

showing RFLP results after restriction of a PCR product.