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The rate limiting step in the formation of 1,25 dihydroxy Vitamin D3 (the active form of the vitamin) is:
the addition of a hydroxyl group in the kidney
the addition of a hydroxyl group in the liver
plasma calcitonin levels
the absorption of precursor (Vitamin D3) from the gastrointestinal tract
the formation of Vitamin D3 in the skin caused by exposure to UV light
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