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Which of the following approaches can be used to generate embryonic stem cells (...

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Which of the following approaches can be used to generate embryonic stem cells (ESCs)?

Transfer of the nucleus of a differentiated cell into an enucleated somatic cell

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Transfer of mature germ cells into cell culture

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Removal of cells of the epiblast from a blastocyst

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Expression of key transcription factors in a somatic cell

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