You find that conditional deletion of the Dlx3 gene in the neural tube of mouse embryos produces a specific neural tube defect at E11.5. What method could you use to rapidly test if the gene has the same or similar function in the chicken embryo?

Question

You find that conditional deletion of the  Dlx3  gene in the neural tube of mouse embryos produces a specific neural tube defect at E11.5. What method could you use to  rapidly test  if the gene has the same or similar function in the chicken embryo?

Answer

Targetted deletion of the  Dlx3  gene in chicken, using ES cells

Answer

In ovo  electroporation of  Dlx3  into the neural tube of early chicken embryos

Answer

In situ  hybridisation for  Dlx3  mRNA expression in chicken embryos

Answer

Electroporation of Cas9 and  Dlx3  guide RNAs into the chicken neural tube

Crowdly

You find that conditional deletion of the Dlx3 gene in the neural tube of mous...

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