The researcher designs a forward and reverse primer pair that specifically bind...
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The researcher designs a forward and reverse primer pair that specifically binds to DNA sequences flanking exon 7 of the APC gene. The primer nucleotide sequences are provided in Table 2.
Table 2. Forward and reverse primer nucleotide sequences
| Primer | Nucleotide sequence (5′→3′) |
|---|---|
| Forward primer | GCCTTGGGCTAAGAAAGCCTA |
| Reverse primer | CAGCACATTGGTACTGAATGCTT |
Q3. Use the equation listed below to calculate and record the melting temperature (Tm) of the forward and reverse primers. Record the Tm to the nearest whole number. (2 marks)
Tm (°C) = 69.4 + (0.41x(%GC)) - (650/N)
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