Q6: Research – Your Experimental Data from the Gut Lab

Submit ONE PDF file that includes all parts of Q6 and Q7.

Part 1: Figures and Figure Legends

Please submit the following two figures of your experimental recordings from the gut lab:

• Figure 2: Protocol A (Nerve Stimulation)
• Figure 3: Atropine + Protocol A (Nerve Stimulation)

Each figure must include a detailed legend [maximum 150 words each].

Part 2: Tables

Include your ‘Nerve Stimulation’ and ‘Acetylcholine’ data tables from the gut lab. Label these table 1 and table 2.

You must show your calculations for all cells in these tables.

Part 3: Analysis of your experimental data 

Referring to the relevant figure(s) and table(s) you have provided, describe what happened to the response to myenteric nerve stimulation after the addition of atropine to the organ bath. EXPLAIN why atropine does not completely inhibit the response to nerve stimulation, but normally results in a near-complete loss of the contractile response to acetylcholine (in the rat ileum). In your response you should describe the pathways following muscarinic receptor activation that led to the increase in the force of contraction. [maximum 250 words]

Q7: Research: Conference Abstract

Exciting news! Your BMS2031 Gut Lab experiments performed for Professor Choate’s drug company (Motilix) will be presented at the annual International Conference for Gut Motility. You must provide an abstract of your experiments for the conference organisers.

Abstracts are short, informative and engaging summaries of experiments. Your abstract must be no more than 400 words and must include:

1. A TITLE: short [max 25 words], informative and engaging (i.e., something that will encourage your audience to continue reading your abstract and to come to your conference presentation!)
2. AN INTRODUCTION: in no more than 100 words, explain the topic. This background is important for the reader to understand the purpose of the research (why did you do the experiment, why is it important, what problem are you trying to solve?)
3. HYPOTHESIS/GENERAL PROBLEM: What specific question did you address? In max 25 words, state your hypothesis OR the general problem being addressed by your study
4. METHODS: in no more than 50 words, describe your experimental set-up and methodology
5. RESULTS: in no more than 100 words, concisely describe your results (you should include some of the data – no figures, just text)
6. DISCUSSION: in no more than 100 words, put your results into the context of the neuronal control of gut motility AND drive home the broader implications of your experimental findings. Include a brief comment on any limitations or future directions of your experiments.

Note: Please do not provide references for this abstract and please keep the headers in your response: title, introduction, etc…

[maximum 400 words for your abstract]

Note: your responses to Q6 parts 1, 2 , 3 & Q7 should ALL be contained within a PDF file and not in the text box.