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During Lab 4 you compared the amount of GFP under the control of four different ...
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During Lab 4 you compared the amount of GFP under the control of four different promoters, named Araπ1, Araπ2, Araπ3 and Araπ4. The following diagram (Figure 1) shows a sequence alignment between Araπ2 and the wildtype promoter construct.
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Figure 1: Alignment between Araπ2 and wildtype promoter constructs. The red boxes identify key sequences necessary for transcription (ie: AraC binding sites and the promoter sequence). Where the wildtype and Araπ2 sequence is identical, a nucleotide base is shown in the consensus sequence. Where there is a difference between the wildtype and the Araπ2 sequence a β+β sign is shown in the consensus sequence.The table below shows the relative fluorescence of the two strains obtained from Lab 4, the number of plus signs β+β gives an indication of the amount of fluorescence (ie: β+++β showed more fluorescence than β++β. A minus sign β-β indicates no fluorescence was observed).
| Induced (supplemented with arabinose) | Uninduced (not supplemented with arabinose) | |
|---|---|---|
| Wildtype | +++ | - |
| Araπ2 (mutant) | ++ | - |